Author
Topic: 1 Kb
doc d
late to the party
posted 18.11.2005 11:15
well its only a small milestone but its big enough to be the cyn-3 promoter. which is excretory cell specific. and not at all interesting. i just didn't want to spunk a thousand on roy keane.
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Ponder
TMO Member
posted 18.11.2005 11:19
quote: Originally posted by doc d: i just didn't want to spunk on roy keane. Roy does. You can just tell . It's kind of creepy. Anyhoo, congrats on the the big 1-0-0-0.
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Roy
Mohammed the Gay Ninja
posted 18.11.2005 11:23
Not as creepy as someone still walking across the messageboard in their 'disguise'. Christ, people even still call you ralph. Well done, doc.
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doc d
late to the party
posted 18.11.2005 11:39
why not abby? have you: gradient? Mg conc? diff taqs? touchdown? hot start? touchdown hot start? optiprime buffers??
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Abby
Slave Girl of Gor
posted 18.11.2005 11:49
No, I can’t be bothered! :sulk: It is just a stupid simple one that has (allegedly) already been optimised etc. It worked a little bit in some lanes once, so I’m going to try more cycles then just make the results up I reckon.
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Abby
Slave Girl of Gor
posted 18.11.2005 11:50
Oh, I have done a hotstart though. It was rubbish. I think I just keep sneezing in it or something.
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doc d
late to the party
posted 18.11.2005 11:54
remember: sometimes less template is better. are you using a good taq? i'm sure you've answered all these yourself. er. wow, i've turned into the wandering post doc of advice....
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Abby
Slave Girl of Gor
posted 18.11.2005 14:07
Yeah, am going to do less template as well. The taq should be fine, it is from a kit with a mystery magical PCR Enhancer!1 Will report back! Now, it is Friday night, I must stop being geeky and go to the pub... [ 18.11.2005, 14:07: Message edited by: Abby ]
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