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Author Topic: 1 Kb
doc d
late to the party
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well its only a small milestone but its big enough to be the cyn-3 promoter. which is excretory cell specific. and not at all interesting.

i just didn't want to spunk a thousand on roy keane.

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Ponder
TMO Member
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quote:
Originally posted by doc d:
i just didn't want to spunk on roy keane.

Roy does. You can just tell. It's kind of creepy.

Anyhoo, congrats on the the big 1-0-0-0.

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Roy
Mohammed the Gay Ninja
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Not as creepy as someone still walking across the messageboard in their 'disguise'. Christ, people even still call you ralph.

Well done, doc.

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Abby
Slave Girl of Gor
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My PCR wont work [Frown]
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doc d
late to the party
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why not abby?
have you:
gradient?
Mg conc?
diff taqs?
touchdown?
hot start?
touchdown hot start?
optiprime buffers??

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Abby
Slave Girl of Gor
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No, I can’t be bothered! :sulk: It is just a stupid simple one that has (allegedly) already been optimised etc. It worked a little bit in some lanes once, so I’m going to try more cycles then just make the results up I reckon.
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Abby
Slave Girl of Gor
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Oh, I have done a hotstart though. It was rubbish. I think I just keep sneezing in it or something.
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doc d
late to the party
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remember:
sometimes less template is better.

are you using a good taq?

i'm sure you've answered all these yourself.

er.
wow, i've turned into the wandering post doc of advice....

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Abby
Slave Girl of Gor
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Yeah, am going to do less template as well. The taq should be fine, it is from a kit with a mystery magical PCR Enhancer!1 Will report back!

Now, it is Friday night, I must stop being geeky and go to the pub...

[ 18.11.2005, 14:07: Message edited by: Abby ]

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